Polyphenols
analysis- A mixture of 34 compounds were analyzed between 100 ng
to 5 μg (dissolved in ACN:H2O 1:4).
For HPLC analysis: Waters (Milford, MA, USA) LC system and 250×4.6
mm i.d., 4 μm reversed-phase Nova-Pak C18 (Waters, Milford, MA,
USA) column.
Gradient of: solvent A (water, NaCl 0.1M/acetic acid, 98:2, v/v)
and solvent B (water/acetonitrile/acetic acid, 73:25:2, v/v/v) at
a flow rate of 1.0 mL/min
(0?80% B linear from 0 to 55 min, 80?90% B linear, from 55 to 57
min, 90% B isocratic from 57 to 70 min, 90?95% B linear from 70
to 80 min,
95?100% B linear from 80 to 90 min). Volume of sample: 40 μL. The
detection conditions were: Amperometric Detection at 1.0 V with
Multi-Walled Carbon
Nanotubes Screen-Printed Electrodes (Ref. 110CNT).
Compounds: 1) 3,4-OH-maldelic ac; 2) 4-OH-mandelic a;c 3) Gallic
a; 4) 1,2,3-OH-bencene; 5) 3-OH-mandelic ac; 6) 3-OH-4-MeO-mandelic
ac;
7) Protocatechuic ac; 8) Pyrocatechol; 9) 3,4-OH-phenylacetic; 10)
4,5-OH-4-MeO-benzoic ac; 11) 4-OH-benzoic ac; 12) 3-(3,4-OH-phenyl)propionic
ac;
13) 4-OH-hippuric ac; 14) Vanillic ac; 15) Caffeic ac; 16) 3-OH-benzoic
ac; 17) 4-OH-3-MeO-phenyl acetic ac; 18) Syringic ac; 19) 4-methylcatechol;
20) 3-OH-phenyl acetic 21) 3-(4-OH-phenyl)propionic ac; 22) 4-OH-coumaric
ac; 23) 3-(3-OH-phenyl)propionic ac; 24) Ferulic ac; 25) Benzoic
ac;
26) Veratric ac; 27) Phenylacetic ac; 28) Homoveratric ac 29) Isoferulic
ac; 30) Homoanisic ac; 31) 4-MeO-benzoic ac; 32) 4-ethylcatechol;
33) 3-(3,4-MeO-phenyl)propionic ac; 34) 3,4,5-MeO-cinnamic ac; acid
(ac), hydroxy (OH), methoxy (MeO).
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